Fabrication of structually comlicated soft tissue such as liver-tissues and understanding its biological function of teh fabricated liver-tissue

We have newly developed heparin-immobilized temperature-responsive cell culture surface (TRCS), further modifying the surfaces with growth factor to fabricate hepatic-cells sheet. We have demonstrated that hepatic-cells sheet was readily fabricated with growth-factor and heparin complex immobilized surface, maintaining physiological function characteristic of hepatic-tissue for longer period. Micro-patterned TRCS was also developed, being applied to fabricate nerve-tissue. The surface would be expected as base technology for fabrication of nerve-tissue. A facile method of preparation of TRCS without special equipment and reagents were newly developed. We also innovated robotics to create robot, which attained precise and multiple micro-contacting, and devise to transplant and/or carry cell-sheet easily for short time. Rapid and convenient method for fabricating soft-tissue such as hepatic and nerve tissues would be attained, combing those techniques.